Cereal biotechnology by Peter C. Morris, James H. Bryce
By Peter C. Morris, James H. Bryce
(Woodhead Publishing constrained) Discusses genetic amendment in cereal biotechnology, explaining the root for present laws and strategies for assessing hazards. additionally addresses particular functions for cereal biotechnology from using molecular organic instruments cereal construction tools.
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Enzymatic digestion of the walls of such cells releases populations of protoplasts (naked cells), which are mixed with foreign DNA. Protoplasts take up this DNA through their plasma membrane when treated with chemicals such as polyethylene glycol (PEG) and/or highvoltage electrical pulses. Several difficulties are associated with this technique. Although about 50% of the protoplasts take up exogenous DNA, the final transformation frequency is low, and is usually in the order of 1 protoplast in 105 being stably transformed.
For wheat, another emphasis will be in starch biosynthesis and dough characteristics, while for barley emphasis might be on malting characteristics and nutritional value. Moreover, both barley and wheat could be used for the production of proteins with high value. On the technological front, improved transformation protocols for wheat and barley will appear. The introduction of transgenes will also be done more carefully, so that no selectable markers are present in the transformants. It might also be possible in the future to transform large DNA molecules which carry information of multiple genes or a complete trait.
29. 39 Improvement of cereal quality by genetic engineering; Guthrie Centenary Conference of the Royal Australian Chemical Institute Cereal Chemistry Division, Sydney, New South Wales, Australia, Plenum Press, New York, 1994. TOURAEV, A, VICENTE, O and HEBERLE-BORS, E, Initiation of microspore embryogenesis by stress. Trends in Plant Science, 1997 2(8) 297–302. ). Genome, 1997 40 570–81. WAN, Y and LEMAUX, P G, ‘Biolistic transformation of microspore derived and immature zygotic embryos and regeneration of fertile transgenic barley plants’, in Gene Transfer to Plants, I.